Forensic Technologies in Music Copyright

The essay explores some recent controversies in British music copyright through the evolving technologies used to perform or play music in the courtroom. While the conceptual tension between cases has caused doctrinal anxiety about the effect of popular music in copyright, the essay contends that the recent stream of music copyright cases can be considered from a historical perspective, taking into account the tools, materials and experts as they featured in court. In doing so, the essay connects a history of legal expertise to the emergence of new technologies while arguing that legal knowledge about music copyright was, in fact, stabilised in the courtroom

Hair organ regeneration via the bioengineered hair follicular unit transplantation

Organ regenerative therapy aims to reproduce fully functional organs to replace organs that have been lost or damaged as a result of disease, injury, or aging. For the fully functional regeneration of ectodermal organs, a concept has been proposed in which a bioengineered organ is developed by reproducing the embryonic processes of organogenesis. Here, we show that a bioengineered hair follicle germ, which was reconstituted with embryonic skin-derived epithelial and mesenchymal cells and ectopically transplanted, was able to develop histologically correct hair follicles. The bioengineered hair follicles properly connected to the host skin epithelium by intracutaneous transplantation and reproduced the stem cell niche and hair cycles. The bioengineered hair follicles also autonomously connected with nerves and the arrector pili muscle at the permanent region and exhibited piloerection ability. Our findings indicate that the bioengineered hair follicles could restore physiological hair functions and could be applicable to surgical treatments for alopecia

Bright Side of Lignin Depolymerization:Toward New Platform Chemicals

Lignin, a major component of lignocellulose, is the largest source of aromatic building blocks on the planet and harbors great potential to serve as starting material for the production of biobased products. Despite the initial challenges associated with the robust and irregular structure of lignin, the valorization of this intriguing aromatic biopolymer has come a long way: recently, many creative strategies emerged that deliver defined products via catalytic or biocatalytic depolymerization in good yields. The purpose of this review is to provide insight into these novel approaches and the potential application of such emerging new structures for the synthesis of biobased polymers or pharmacologically active molecules. Existing strategies for functionalization or defunctionalization of lignin-based compounds are also summarized. Following the whole value chain from raw lignocellulose through depolymerization to application whenever possible, specific lignin-based compounds emerge that could be in the future considered as potential lignin-derived platform chemicals

Liver cell therapy: is this the end of the beginning?

The prevalence of liver diseases is increasing globally. Orthotopic liver transplantation is widely used to treat liver disease upon organ failure. The complexity of this procedure and finite numbers of healthy organ donors have prompted research into alternative therapeutic options to treat liver disease. This includes the transplantation of liver cells to promote regeneration. While successful, the routine supply of good quality human liver cells is limited. Therefore, renewable and scalable sources of these cells are sought. Liver progenitor and pluripotent stem cells offer potential cell sources that could be used clinically. This review discusses recent approaches in liver cell transplantation and requirements to improve the process, with the ultimate goal being efficient organ regeneration. We also discuss the potential off-target effects of cell-based therapies, and the advantages and drawbacks of current pre-clinical animal models used to study organ senescence, repopulation and regeneration

Profiling the Impact of Medium Formulation on Morphology and Functionality of Primary Hepatocytes in vitro

The characterization of fully-defined in vitro hepatic culture systems requires testing of functional and morphological variables to obtain the optimal trophic support, particularly for cell therapeutics including bioartificial liver systems (BALs). Using serum-free fully-defined culture medium formulations, we measured synthetic, detoxification and metabolic variables of primary porcine hepatocytes (PPHs) - integrated these datasets using a defined scoring system and correlated this hepatocyte biological activity index (HBAI) with morphological parameters. Hepatic-specific functions exceeded those of both primary human hepatocytes (PHHs) and HepaRG cells, whilst retaining biotransformation potential and in vivo-like ultrastructural morphology, suggesting PPHs as a potential surrogate for PHHs in various biotech applications. The HBAI permits assessment of global functional capacity allowing the rational choice of optimal trophic support for a defined operational task (including BALs, hepatocellular transplantation, and cytochrome P450 (CYP450) drug metabolism studies), mitigates risk associated with sub-optimal culture systems, and reduces time and cost of research and therapeutic applications

Development of Corn Stover Biofuel: Impacts on Corn and Soybean Markets and Land Rotation

This paper first develops a partial equilibrium (PE) model to examine impacts of converting corn stover to biofuel on markets for corn and soybeans at the national market level. The PE model links gasoline, corn ethanol, dried distiller grains, corn, soybeans, and soybean meal markets in the presence and absence of a viable market for corn stover. The model also includes a technology which converts corn stover to bio-gasoline (a drop-in biofuel). The model evaluates profitability of the ethanol and bio-gasoline industries and assumes that these industries will expand/contract until profits reach zero. Given these assumptions and according to the predetermined supply and demand elasticities, the model determines equilibrium prices and their corresponding quantities for given exogenous variables defined in the model (such as crude oil price). The model is calibrated using data obtained for 2010 for USA economy and then solved for alternative crude oil prices in the presence and absence of a fixed subsidy of $1.01per gallon of bio-gasoline produced. Then we used the Purdue Crop Linear Programing (PCLP) model to assess farmers’ reactions to market equilibrium prices for corn, soybeans, and corn stover in the presence of a viable market for corn stover. The PCLP model determines profit-maximizing decisions for a given farm given its existing resources and estimated prices of commodities and input costs. We tuned the PCLP model according to the market clearing prices obtained from the PE model for a case when the crude oil price is$100 per barrel. Then using the tuned PCLP model we determined the optimum land allocation options for farmers. The partial equilibrium analyses show that: 1) with no bio-gasoline subsidy a limited amount of corn stover will be converted to biofuel even at very high crude oil prices; 2) The bio-gasoline subsidy could significantly boost production of this biofuel in particular at medium and higher crude oil prices; 3) no more than 45% of available corn stover will be removed for biofuel production; 4) converting corn stover to bio-gasoline boosts corn production, increases corn-corn rotation, and decreases supply of soybeans; and 5) converting corn stover to bio-gasoline changes the soybean to corn price ratio in favor of soybeans, at least in the very short term. The results obtained from the PCLP model show that the farm level land allocation decision is sensitive to the profitability of corn stover processing activities. When corn stover removal is introduced as a new option under the base case scenario at a corn stover price of $111 per ton) farmers allocate about 66% of their land to the corn-corn rotation and remove stover from their land. In this case corn stover is removed from 78.2% of available land at a rate of 1.18 tons per acre. If corn stover is demanded for biofuel production, then a major shift will be observed in crop rotations

Development of Corn Stover Biofuel: Impacts on Corn and Soybean Markets and Crop Rotation

What would be the impacts of a viable market for corn stover? A partial equilibrium model and a linear programing model were used to determine to what extent the existence of a viable market for corn stover would affect the traditional corn-soybean crop rotation in the US. We find that with government support production of biofuel from corn stover could significantly increase. That boosts profitability of farming corn in combination with harvesting corn stover versus soybeans. We show that if corn stover is demanded for biofuel, then a major shift will be observed in crop rotations in the US